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control shrna lentiviral particles  (OriGene)


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    Structured Review

    OriGene control shrna lentiviral particles
    Control Shrna Lentiviral Particles, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+shrna+lentiviral+particles/Chymotrypsin+like+protease+(CTRL)+Human+shRNA+Lentiviral+Particle/pm42127012-61-7-18
    Average 94 stars, based on 1 article reviews
    control shrna lentiviral particles - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Interplay between Leptin and Stearoyl-CoA Desaturase 1 in Estrogen Receptor-Positive Breast Cancer Cells.
    Article Snippet: .. Lentiviral Transfection The establishment of a stable MCF-7 cell line has been achieved by using control shRNA lentiviral particles (TR30021V; OriGene Technologies, Rockville, MD) and SCD shRNA lentiviral particles (TL309631V; OriGene Technologies) following manufacturer’s instructions. ..

    Control:

    Article Title: Interplay between Leptin and Stearoyl-CoA Desaturase 1 in Estrogen Receptor-Positive Breast Cancer Cells.
    Article Snippet: .. Lentiviral Transfection The establishment of a stable MCF-7 cell line has been achieved by using control shRNA lentiviral particles (TR30021V; OriGene Technologies, Rockville, MD) and SCD shRNA lentiviral particles (TL309631V; OriGene Technologies) following manufacturer’s instructions. ..

    Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells.
    Article Snippet: .. The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies). ..

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer
    Article Snippet: .. SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher ® Scientific).

    Article Title: KIF11 promotes rheumatoid arthritis pathogenesis by activating M1 macrophage polarization and promoting inflammatory cytokine secretion
    Article Snippet: .. The transduction of both KIF11 shRNA and control shRNA lentiviral particles was conducted following the protocol provided by Origene and as described [ ]. ..

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific).

    Article Title: Long non-coding RNA H19 enhances the pro-apoptotic activity of ITF2357 (a histone deacetylase inhibitor) in colorectal cancer cells.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 HCT-116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (Cat. n° TL318197V, OriGene Technologies, Inc., Rockville, MD, United States), while relative control cells were infected with control shRNA lentiviral particles (Cat. n° TR30021V, OriGene Technologies, Inc., Rockville, MD, United States). .. Subsequently, infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center) and maintained in culture under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific, United States).

    shRNA:

    Article Title: Interplay between Leptin and Stearoyl-CoA Desaturase 1 in Estrogen Receptor-Positive Breast Cancer Cells.
    Article Snippet: .. Lentiviral Transfection The establishment of a stable MCF-7 cell line has been achieved by using control shRNA lentiviral particles (TR30021V; OriGene Technologies, Rockville, MD) and SCD shRNA lentiviral particles (TL309631V; OriGene Technologies) following manufacturer’s instructions. ..

    Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells.
    Article Snippet: .. The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies). ..

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer
    Article Snippet: .. SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher ® Scientific).

    Article Title: KIF11 promotes rheumatoid arthritis pathogenesis by activating M1 macrophage polarization and promoting inflammatory cytokine secretion
    Article Snippet: .. The transduction of both KIF11 shRNA and control shRNA lentiviral particles was conducted following the protocol provided by Origene and as described [ ]. ..

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific).

    Article Title: Long non-coding RNA H19 enhances the pro-apoptotic activity of ITF2357 (a histone deacetylase inhibitor) in colorectal cancer cells.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 HCT-116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (Cat. n° TL318197V, OriGene Technologies, Inc., Rockville, MD, United States), while relative control cells were infected with control shRNA lentiviral particles (Cat. n° TR30021V, OriGene Technologies, Inc., Rockville, MD, United States). .. Subsequently, infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center) and maintained in culture under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific, United States).

    Transduction:

    Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells.
    Article Snippet: .. The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies). ..

    Article Title: KIF11 promotes rheumatoid arthritis pathogenesis by activating M1 macrophage polarization and promoting inflammatory cytokine secretion
    Article Snippet: .. The transduction of both KIF11 shRNA and control shRNA lentiviral particles was conducted following the protocol provided by Origene and as described [ ]. ..

    Article Title: KIF11 promotes rheumatoid arthritis pathogenesis by activating M1 macrophage polarization and promoting inflammatory cytokine secretion.
    Article Snippet: .. The transduction of both KIF11 shRNA and control shRNA lentiviral particles was conducted following the protocol provided by Origene and as described [14]. ..

    Stable Transfection:

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer
    Article Snippet: .. SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher ® Scientific).

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific).

    Article Title: Long non-coding RNA H19 enhances the pro-apoptotic activity of ITF2357 (a histone deacetylase inhibitor) in colorectal cancer cells.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 HCT-116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (Cat. n° TL318197V, OriGene Technologies, Inc., Rockville, MD, United States), while relative control cells were infected with control shRNA lentiviral particles (Cat. n° TR30021V, OriGene Technologies, Inc., Rockville, MD, United States). .. Subsequently, infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center) and maintained in culture under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific, United States).

    Article Title: Long non-coding RNA H19 enhances the pro-apoptotic activity of ITF2357 (a histone deacetylase inhibitor) in colorectal cancer cells
    Article Snippet: .. HCT-116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (Cat. n° TL318197V, OriGene Technologies, Inc., Rockville, MD, United States), while relative control cells were infected with control shRNA lentiviral particles (Cat. n° TR30021V, OriGene Technologies, Inc., Rockville, MD, United States). .. Subsequently, infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center) and maintained in culture under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher ® Scientific, United States).

    Infection:

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer
    Article Snippet: .. SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher ® Scientific).

    Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 SW620 and HCT116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (cat. n° TL318197V, OriGene Technologies, Inc., United States), relative control cells were infected with control shRNA lentiviral particles (cat. n° TR30021V, OriGene Technologies, Inc., United States). .. The infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center, Italy) and maintained under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific).

    Article Title: Long non-coding RNA H19 enhances the pro-apoptotic activity of ITF2357 (a histone deacetylase inhibitor) in colorectal cancer cells.
    Article Snippet: .. Infection with lentiviral vectors to stably silence lncH19 HCT-116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (Cat. n° TL318197V, OriGene Technologies, Inc., Rockville, MD, United States), while relative control cells were infected with control shRNA lentiviral particles (Cat. n° TR30021V, OriGene Technologies, Inc., Rockville, MD, United States). .. Subsequently, infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center) and maintained in culture under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher® Scientific, United States).

    Article Title: Long non-coding RNA H19 enhances the pro-apoptotic activity of ITF2357 (a histone deacetylase inhibitor) in colorectal cancer cells
    Article Snippet: .. HCT-116 cells were stably silenced for lncH19 by lentiviral infection with H19 human shRNA lentiviral particles (Cat. n° TL318197V, OriGene Technologies, Inc., Rockville, MD, United States), while relative control cells were infected with control shRNA lentiviral particles (Cat. n° TR30021V, OriGene Technologies, Inc., Rockville, MD, United States). .. Subsequently, infected cells were selected by cell sorting (BD FACSAriaTM III Sorter, ATeN Center) and maintained in culture under selective pressure with 1 mg/mL of puromycin (GibcoTM puromycin dihydrochloride, cat. n°A1113802, Thermo Fisher ® Scientific, United States).



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    Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
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    Image Search Results


    Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle

    doi: 10.1093/ajrcmb/aanag058

    Figure Lengend Snippet: Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

    Article Snippet: Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA).

    Techniques: CyQUANT Assay, Comparison, Expressing, shRNA, Knockdown, Western Blot, Analysis